Categories: Guest posts , Life in the lab |  Comments
Guest post by Heather Cassell
In the lab, you develop a fondness for working with certain things: compliant equipment, pleasant smelling solvents, easy-to-culture bacteria. One of my favourites are fluorescent proteins – their bright colours can make even the dullest day that little bit more cheery. I find them a joy to work with not only because of their beauty, but because the source of that beauty also makes them easy to work with.
A good example of this is in protein production. During expression in E. coli, you often cannot tell how well expression of a colourless protein is going, but because fluorescent proteins will produce a colour even at a relatively low concentrations, it can be seen while the cells are still growing. This allows you to keep track of your progress, answering key questions like: do I have any protein? Or did I add the chemical I need to produce the protein? (The latter being a not uncommon mistake for a sleep-deprived scientist.) Getting answers to these visually means no lengthy purification procedure, avoiding the inevitable disappointment.
The colouration continues to be helpful as you go through the protein purification process: you can easily see if your protein has been released from the cells, whether it has bound to the column, if it has been released from the column and so on. Again, each of these steps requires another means of detection in colourless proteins. (more…)